Funny Images Conjured up by Web Comments

Funny Images Conjured up by Web Comments Funny Images Conjured up by Web Comments Funny Images Conjured up by Web Comments By Maeve Maddox Sometimes Im more amused than annoyed by spelling errors and incorrect word choices that I see in blogs and comments. Here are a few. Let your imagination soar! 1. Looking for a laptop for my mother she only needs a bear-bones laptop. 2. Someone said this to me one time and I balled my eyes out. 3. his scarlet bishops cossack and cap. 4. The boy [who had been beaten] had whelps on him. 5. He hears a disemboweled voice. 6. The cowboy was rounding up the doggies. 7. The crust of my interest is World War I. 1. bare-bones adjective meaning essential. a laptop with only the most essential features. bear-bones the skeletal structure of a bear (an animal of the family Ursidae). 2. balled formed into a ball. We can speak of a balled fist. Yarn can be balled, as can little bits of wool on a sweater. bawled past tense of bawl, to cry out loudly. The word may come from an Icelandic word for the sound cattle make. Related to bellow. 3. Cossack originally a member of a Russian military elite; a distinctive item of their uniform was a tall fur hat. Figuratively, a cossack is an authoritarian figure that uses any type of force to control others. The character Chekov in the original StarTrek series was fond of calling people he didnt like cossacks. A cassock, on the other hand, is a clerical garment, a long close-fitting tunic reaching to the feet. This is what the bishop probably had. 4. A whelp is the young of a carnivorous animal, such as a wolf cub or puppy. The word called for in this context is welt. welt: a raised area, ridge, or seam on the body surface (as from scarring or a blow). 5. disembowel: to take out the bowels of, eviscerate. This is what the word drawn refers to in the expression hanged, drawn, and quartered. The word this writer was reaching for was disembodied. disembodied in this context means that a voice was heard, but its source could not be seen. 6. doggies a childs word for dogs. Ex. Look at the Mother Doggy and all the little doggies! dogy (also spelled dogey and dogie) a motherless calf in a range herd. 7. crust the hardened exterior of something. It could be a pie crust or the earths crust. The speaker probably intended to say crux. crux a word derived from the Latin word for cross. A cross, as we know, can be a tool of torture and execution, but its shape is also suggestive of a central nexus, like a crossroads. Both ideas contribute to the meanings of the English word crux: 1 a. a puzzling, confusing, or difficult problem : an unsolved question b. a determinative point at issue : a pivotal or essential point requiring resolution or resolving an outcome 2. a main or central feature (as of an argument or plan) Please share your own examples of misused words that conjure up funny images. Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Misused Words category, check our popular posts, or choose a related post below:5 Uses of InfinitivesLatin Plural Endings30 Words for Small Amounts

Phosphate-Buffered Saline or PBS Solution

Phosphate-Buffered Saline or PBS Solution PBS or phosphate-buffered saline is a buffer solution that is particularly valuable because it mimic the ion concentration, osmolarity, and pH of human body fluids. In other words, its isotonic to human solutions, so its less likely to cause cell damage, toxicity,  or unwanted precipitation in biological, medical, or biochemical research. PBS Chemical Composition There are several recipes to prepare PBS solution. The essential solution contains water, sodium hydrogen phosphate, and sodium chloride. Some preparations contain potassium chloride and potassium dihydrogen phosphate. EDTA may also be added in cellular preparation to prevent clumping. Phosphate-buffered saline is not ideal for use in solutions that contain divalent cations (Fe2, Zn2) because precipitation may  occur. However, some PBS solutions do contain calcium or magnesium. Also, keep in mind phosphate may inhibit enzymatic reactions. Be particularly aware of this potential disadvantage when working with DNA. While PBS is excellent for physiological science, be aware the phosphate in a PBS-buffered sample may precipitate if the sample is mixed with ethanol. A typical chemical composition of 1X PBS has a final concentration of  10 mM PO43−, 137 mM NaCl, and 2.7 mM KCl. Heres the final concentration of reagents in the solution: Salt Concentration (mmol/L) Concentration (g/L) NaCl 137 8.0 KCl 2.7 0.2 Na2HPO4 10 1.42 KH2PO4 1.8 0.24 Protocol  for Making Phosphate-Buffered Saline Depending on your purpose, you may prepare 1X, 5X, or 10X PBS. Many people simply purchase PBS buffer tablets, dissolve them in distilled water, and adjust the pH as needed with hydrochloric acid or sodium hydroxide. However, its easy to make the solution from scratch. Here are recipes for 1X and 10X phosphate-buffered saline: Reagent Amountto add (1) Final concentration (1) Amount to add (10) Final concentration (10) NaCl 8 g 137 mM 80 g 1.37 M KCl 0.2 g 2.7 mM 2 g 27 mM Na2HPO4 1.44 g 10 mM 14.4 g 100 mM KH2PO4 0.24 g 1.8 mM 2.4 g 18 mM Optional: CaCl22H2O 0.133 g 1 mM 1.33 g 10 mM MgCl26H2O 0.10 g 0.5 mM 1.0 g 5 mM Dissolve the reagent salts in 800 ml distilled water.Adjust the pH to the desired level with hydrochloric acid. Usually this is 7.4 or 7.2. Use a pH meter to measure the pH, not pH paper or other imprecise technique.Add distilled water to achieve a final volume of 1 liter. Sterilization and Storage of PBS Solution Sterilization isnt necessary for some applications, but if your are sterilizing it, dispense the solution into aliquots and autoclave for 20 minutes at 15 psi (1.05 kg/cm2) or use filter sterilization. Phosphate-buffered saline may be stored at room temperature. It may also be refrigerated, but 5X and 10X solution may precipitate when cooled. If you must chill a concentrated solution, first store it at room temperature until you are certain the salts have completely dissolved. If precipitation does occur, warming the temperature will bring them back into solution. Shelf life of refrigerated solution is 1 month. Diluting a 10X Solution to Make 1X PBS 10X is a concentrated or stock solution, which may be diluted to make a 1X or normal solution. A 5X solution must be diluted 5 times to make a normal dilution, while a 10X solution must be diluted 10 times. To prepare a 1 liter working solution of 1X PBS from a 10X PBS solution, add 100 ml of the 10X solution to 900 ml of water. This only changes the concentration of the solution, not the gram or molar amount of the reagents. The pH should be unaffected.   PBS Versus DPBS Another popular buffer solution is Dulbeccos phosphate buffered saline or DPBS. DPBS, like PBS, is used for biological research and buffers in the 7.2 to 7.6 pH range. It can be stored at room temperature. Dulbeccos solution contains a lower concentration of phosphate. It is 8.1 mM mM phosphate ions, while regular PBS is 10 mM phosphate.  The recipe for 1x DPBS is: Reagent Amount to add (1x) NaCl 8.007 g KCl 0.201 g Na2HPO4 1.150 g KH2PO4 0.200 g Optional: CaCl22H2O 0.133 g MgCl26H2O 0.102 g Dissolve the salts in 800 mL of water. Adjust pH to 7.2 to 76 using hydrochloric acid. Adjust the final volume to 1000 mL with water. Autoclave at 121 °C for 20 minutes. Sources Dulbecco, R.; et al. (1954). Plaque formation and isolation of pure lines with poliomyelitis viruses. J. Exp. Med. 99 (2): 167–182.Phosphate-buffered saline (PBS. Cold Spring Harbor Protocols (2006). Cold Spring Harbor Laboratory Press.